摘要: 葡萄糖氧化酶（GOx）在化学、制药、纺织和其他生物技术工业中有许多新的应用。本研究旨在优化烟曲霉 AFS4 的生产葡萄糖氧化酶。从尼日利亚恩苏卡大学教职工宿舍的花园土壤中分离烟曲霉，并筛选其葡萄糖氧化酶生产能力。通过生化测试和 18S-rDNA 测序确认分离物为烟曲霉。分离菌株被标记为烟曲霉 AFS4。在深层发酵系统中，烟曲霉 AFS4 生产 GOx，酶活力为 1591Uμmol/min，蛋白质浓度为 3.89mg/ml。对 GOx 的生产条件进行了优化，包括碳源、氮源、CaCO3、pH 和发酵时间。葡萄糖（80g/L）是葡萄糖氧化酶（GOx）生产的最佳碳源，其活性为1542μmol/min。与其他测试的氮源相比，蛋白胨（3g/L）更适合于GOx的生产，其活性为1231μmol/min。在生产培养基中添加30g/L的碳酸钙（CaCO3）可提高GOx的产量。GOx生产的最佳pH值为6.5。在第7天，GOx产量达到最高，活性为1517μmol/min。结果证明，烟曲霉AFS4具有生产葡萄糖氧化酶用于各种工业应用的强大潜力。

Abstract: Glucose oxidase (GOx) has several novel applications in chemical, pharmaceutical, textile, and other biotechnological industries. This study was aimed at optimizing the production of glucose oxidase from Aspergillus fumigatus AFS4. Aspergillus fumigatus was isolated from garden soil, obtained from staff quarters University of Nigeria, Nsukka and was screened for glucose oxidase production capability. Biochemical tests and 18S-rDNA sequencing were used to confirm the isolate as Aspergillus fumigatus. The isolate strain was tagged as Aspergillus fumigatus AFS4. GOx was produced from Aspergillus fumigatus AFS4 under submerged fermentation system with an enzyme activity of 1591Uμmol/min and protein concentration of 3.89mg/ml. Different conditions for GOx production which include carbon sources, nitrogen sources, CaCO3 , pH and fermentation time were optimized. Glucose (80g/L) was found to be the best carbon source for GOx production with GOx activity of 1542μmol/min. Peptone (3g/L) was found suitable for GOx production with GOx activity of 1231μmol/min compared to other nitrogen sources tested. CaCO3 enhanced GOx production when 30g/L of it was used to supplement the production medium. The optimum pH for GOx production was 6.5. The highest GOx production was obtained on the 7th day with GOx activity of 1517μmol/min. The results proved that Aspergillus fumigatus AFS4 has strong potential to produce glucose oxidase for various industrial applications.